Precision Immuno-oncology with Nanostring Digital Spatial Profiling (DSP) Technology
Precise detection of multiplex RNA or protein targets in a single run whilst maintaining spatial information across a heterogeneous tissue sample. Gain deeper understanding of the tumour and tumour microenvironment with multiplex gene expression and protein analysis from distinct regions of interest from a whole slide section. This highly multiplexed assay can detect and measure up to 800 analytes in a single section
Key applications in immuno-oncology and Neuroscience research
Morphology driven high-plex profiling on a single tissue section
- Highly multiplexed RNA or protein detection - up to 800 targets in a single run
- RNA or protein analysis from a single FFPE section
- Morphological context - whole slide 4 colour imaging to guide profiling and ROI selection
- Maintains sample integrity - samples can be reused
- Digital quantitation - up to 6 logs (base 10) dynamic range
- High resolution - from 600 micron regions of interest down to single-cell analysis
- High throughput: up to 20 sections per day
Simple and rapid workflow
DSP technology builds upon the highly robust nCounter digital barcoding technology from Nanostring. A simple and fast workflow delivers accurate, reproduceable results for immuno-oncology research.
Precision analysis of any tissue region
from whole tissue to single cell
Up to 4 morphology fluorescent markers can guide selection of regions of interest. Versatile regions of interest from whole tissue, geometric areas or single cell regions can be selected.
Each region of interest is analysed on the nCounter platform providing counts for each individual analyte. Once all regions of interest have been analysed, raw data can be provided in an excel document along with normalised data in graphical form including heatmaps to provide increased data insights.
Raw data is provided in the form of an excel data sheet, and images include whole tissue images at low magnification and individual ROI at high magnification from all samples. Power point documentation and DSP App (a live data visualization tool) will be provided to customers.
Stained sections can be archived on site in a dedicated, secure archive facility and remaining tissue blocks will be returned to the customer. Data will be archived for 12 months after completion of the study.
Data Quality Control
The use of 6 positive and negative control probes ensure hybridisation efficiency, prep station purification and imaging are at optimum levels. Assay variation is controlled and monitored using control targets built into the CodeSet reagents.
High reproducibility and correlation of Positive Control Counts vs. protein tag set concentration. Nanostring assays provide a correlation of positive control counts to Protein tag target concentration with R2>0.95.
Digital Spatial profiling services at Propath provide powerful insights for many key applications including Immuno-Oncology and Neuroscience research. Contact us to discuss your study requirements and how Propath’s DSP service can add new insights into your research projects.